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KMID : 0614620020390010022
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Korean Journal of Gastroenterology
2002 Volume.39 No. 1 p.22 ~ p.32
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Long-term Evaluation of Mouse Model Infected with Helicobacter pylori and Influence of Helicobacter pylori Infection on Gastric Carcinogenesis
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Kim Sung-Woo
Cha Choong-Keun
Han Sang-Uk
Kim Young-Bae
Lee Won-Heung
Joo Hee-Jae
Cho Yong-Kwan
Cho Sung-Won
Kim Myung-Wook
Hahm Ki-Baik
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Abstract
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Background/Aims: This study was aimed to evaluate the long-term outcome of Helicobacter pylori (H. pylori)
infection in mouse model and to know the influence of H. pylori infection on gastric carcinogenesis.
Methods: Four-week-old specific pathogen free C57BL/ 6 mice (n=115) were infected with SS1, the mouse-adapted H. pylori strain. In the 4, 8, 16, 24, 36, 50 and 80 weeks after the bacterial inoculation, the H. pylori-infected mice were sacrificed.
Results: After 80 weeks of infection, most of H. pylori-infected mice developed hyperplastic gastritis, but did
not show any evidence of adenoma, dysplasia or carcinoma. Proliferating cell nuclear antigen-positive cells were most abundant at 50 weeks and tended to decrease at 80 weeks. Apoptosis was noted at 8 weeks after H. pylori infection, showing 7-8 apoptotic cells/high power field and tended to increase with the lapse of time. Normally observed neutral mucin was decreased with the lapse of time and it was remarkably decreased at 50 weeks after H. pylori infection. However, acidic mucin was remarkably noted from 50 weeks after the infection.
Conclusions: SS1-infected mice seem to be suitable for H. pylori-related research and H. pylori itself does not induce gastric cancer in normal wild-type mice model in a long-term study, which could be explained by the balance between cell proliferation and apoptosis.
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KEYWORD
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Helicobacter pylori, C57BL/ 6 mice, Gastric carcinogenesis, Apoptosis, Cell proliferation
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